AntiCoV-ID IgG Quantitative ELISA Assay Kit was developed and manufactured in the US by Akston Biosciences
Size: 1×96 wells
Dynamic Range: 3.28-2000 ng/mL
Incubation Time: 2.5 Hours
Sample Type: Serum and Plasma
Species Sample: Human
Sample Size: 10 µL
Alternative Names: Serology, Coronavirus, COVID-19, Corona, Serological
IVD, CE-Marked
Controls Included
Panels were studied with a minimum of eight confirmed disease state samples with this serology assay. No interference was observed for the following disease or infectious agents:
Akston Biosciences’ AntiCoV-ID™ Quantitative IgG ELISA is an indirect, enzyme linked immunosorbent assay (ELISA) designed to measure anti-spike protein receptor binding domain (RBD) IgG antibodies against the SARS-CoV-2 virus (COVID-19 virus, 2019 Novel Coronavirus) in human patient serum and plasma samples, including heat-inactivated serum or heat-inactivated plasma. The indirect immunoassay uses a recombinant SARS-CoV-2 spike protein RBD immobilized on ELISA plates as the capture antigen to bind the SARS-CoV-2 specific anti-spike RBD antibodies in the serum samples when incubated in the microplate wells. A simple wash step removes all unbound proteins, leaving the anti-SARS-CoV-2 spike protein RBD antibodies bound to the plate. A second incubation is performed where the anti-spike protein RBD antibodies are detected by an anti-human IgG antibody (not cross-reactive to IgM) conjugated to horseradish peroxidase (HRP). After a second simple wash step to remove the unbound enzyme-conjugate, the assay plate wells are incubated with 3,3’,5,5’-trimethylbenzidine which causes a colorimetric change that is proportional to the amount of bound enzyme conjugate in each well. The color development is stopped by adding acid that halts development, and the color density of each well is measured using a spectrophotometric microplate reader.
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EagleBio在人类中,冠状病毒会引起呼吸道感染。冠状病毒由几种蛋白质组成,包括刺突(S),包膜(E),膜(M)和核衣壳(N)。结果表明,刺突蛋白与血管紧张素转化酶2(ACE2)受体保持足够的亲和力,可将其用作细胞进入的机制。人们主要认为冠状病毒在人与人之间的传播是通过打喷嚏和咳嗽产生的呼吸道飞沫在亲密接触者之间发生的。IgG是响应抗原产生的最丰富的免疫球蛋白,在长期暴露后会保持在体内。Eagle Biosciences仅提供最优质的测定以满足您的需求,并在这些艰难的时期内为您提供最佳的客户服务。