easYmer HLA-B*35:01 MHC Tetramers Kit

The easYmer HLA-B*35:01 MHC Tetramers Kit is for Research Use Only

Materials Included in Kit:

• easYmer: HLA-B*35:01 – peptide receptive, biotinylated in Tris/Maleate pH 7 with 30% Glycerol
• Folding Buffer: Tris/Maleate pH 7
• Peptide: YF NS2A4–13 (HAVPFGLVSM), a known HLA-B*35:01 binder
  Positive control for evaluation analysis of peptide-HLA folding

Available Sizes:

• 50 Tests (Standard Size)
• 150 Tests
• 500 Tests

Key Benefits of easYmer® MHC Tetramers

• Ready-to-use
• One step loading
• Completely flexible and customizable*
• Biotinylated
• No special equipment needed
• Long shelf-life

*For Custom Tetramer Production, please Contact Us for more information

Scientific Principle

This protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the ß₂-microglobulin (ß₂m? light chain subunit of recombinant HLA Class I (HLA-I) complexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labeled with PE-conjugated anti-human ß₂m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.

Related Products

easYmer HLA-C*07:02 MHC Tetramers Kit
easYmer HLA-B*44:02 MHC Tetramers Kit
easYmer HLA-A*11:01 MHC Tetramers Kit

Product Developed and Manufactured by immunAware

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