easYmer HLA-A*30:02 MHC Tetramers Kit

easYmer HLA-A*30:02 MHC Tetramers Kit Developed and Manufactured by immunAware

Materials Included:

The easYmer kit contains a peptide receptive preparation of HLA, a folding buffer, a positive control peptide for HLA-complex folding. The exact identities of these components are given below:

• easYmer: HLA-A*30:02 – peptide receptive and biotinylated HLA-A*30:02 molecules in Tris/Maleate pH7 with 30% Glycerol
• Folding Buffer: Tris/Maleate pH7
• Peptide: YF NS5 286-295 (KSEYMTSWFY), a known HLA-A*30:02 binder. Positive control for evaluation analysis of peptide-HLA folding

for Research Use Only

Available Sizes:

• 20 Tests (Sample-size)
• 50 Tests (Standard Size)
• 150 Tests
• 500 Tests

Key Benefits of easYmer® MHC Tetramers

• Ready-to-use
• One step loading
• Completely flexible and customizable*
• Biotinylated
• No special equipment needed
• Long shelf-life

*For Custom Tetramer Production, please Contact Us for more information

Assay Principle

This protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the ß₂-microglobulin (ß₂m? light chain subunit of recombinant HLA Class I (HLA-I) comlexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labeled with PE-conjugated anti-human ß₂m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.

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