Hydroxypyridinium Crosslinks HPLC Column

The Hydroxypyridinium Crosslinks HPLC Column is For Research Use Only

Each column is can be utilized for approximately 500-1000 samples depending on column care and sample type.

Assay Background

Pyridinium and deoxypyridinium are built as intramolecular crosslinkers during the maturation of collagen. During bone resorption collagen is degraded and the pyridinium-crosslinks are excreted. They are not re-utilized by the body and excreted in the urine. The crosslinks are not released during the de novo synthesis of collagen. They are specific for resorptive processes. Neither PYD nor DPD are taken up by nutrition. Therefore the determination of the crosslinks is independent from the diet. PYD is found in cartilage, ligaments and bone, whereas DPD is rather specific for bone.

The crosslink excretion shows a strong circadian rhythm with a maximum in the early morning hours. Reliable data can be obtained with a 24h or a creatinine corrected morning urine. An elevated crosslink excretion is shown in osteoporotic women. Crosslink levels decrease during treatment with estrogen (Seibel et al. 1994). Therefore they are suited for therapy monitoring (Ganero et al. 1994). The measurement of pyridinium crosslinks is an independent predictor for future bone fractures (Ganero et al. 1995). Increased crosslink concentrations are also described in patients with Paget’s disease, primary hyperparathyroidism and bone metastasis.

Hydroxypyridinium Crosslinks HPLC Assay kit application for the pyridinium crosslinks makes it possible to determine pyridinium and deoxypyridinium in an easy, fast and precise method. The Hydroxypyridinium Crosslinks HPLC Assay kit includes all reagents in a ready to use form for preparation and separation of the samples with exception of the columns (IC3201rp) and the controls (IC3201ko). Both can be supplied separately by Eagle Biosciences. Beside the complete test kits it is possible to order all components separately. Please request our single component price list.

Related Products

Hydroxypyridinium Crosslinks HPLC Assay
Hydroxypyridinium Crosslinks HPLC Control Set
Ubiquinone Coenzyme Q10 HPLC Column

EagleBio在人类中，冠状病毒会引起呼吸道感染。冠状病毒由几种蛋白质组成，包括刺突（S），包膜（E），膜（M）和核衣壳（N）。结果表明，刺突蛋白与血管紧张素转化酶2（ACE2）受体保持足够的亲和力，可将其用作细胞进入的机制。人们主要认为冠状病毒在人与人之间的传播是通过打喷嚏和咳嗽产生的呼吸道飞沫在亲密接触者之间发生的。IgG是响应抗原产生的最丰富的免疫球蛋白，在长期暴露后会保持在体内。Eagle Biosciences仅提供最优质的测定以满足您的需求，并在这些艰难的时期内为您提供最佳的客户服务。