Aflatoxin HPLC Immunoaffinity Column

The Aflatoxin HPLC Immunoaffinity Column is For Research Use Only

Size: 3ml, 10 columns

Product Developed and Manufactured in Germany by BioTeZ Berlin-Buch GmbH

Principle

This instruction of Aflatoxin total determination in food and feed focuses on the enrichment step of extract using immunoaffinity column (Immunoaffinity Chromotography (IAC)) in combination with quantification with HPLC (High Performance Liquid Chromotography). Accepted laboratory extraction methods should be followed throughout the procedure. If the criteria of organic solvent tolerance, elution process of analyte, and working range of column are followed you will see full performance with the IAC column. If problematic matrices are applied, many pretreatment methods of aflatoxin (AFG2, AFG1, AFB2 and AFB1) determination in food and feed will show low sensitivity because of interfering substances. This method of content determination of aflatoxins combines the high selectivity of an immunoaffinity column (IAC) with its potential to concentrate eluate with the additional step of purification by HPLC column. Please notice that this instruction focuses on the handling with the IAC column. For the commodity extraction step please note that a literature method is provided. The given apparatus (e.g. HPLC system) might serve as example among other possibilities.

Learn more about our Immunoaffinity Columns at Eagle Biosciences’ Product Highlights page dedicated to Immunoaffinity here:

Immunoaffinity Columns: Product Highlights

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